Apr 18, 2025

Public workspaceTriglycerides are an important fuel reserve for synapse function in the brain

DOI
dx.doi.org/10.17504/protocols.io.5qpvooqozv4o/v1
  • 1Weill Cornell Medical College
Mukesh Kumar
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DOI: dx.doi.org/10.17504/protocols.io.5qpvooqozv4o/v1
Collection Citation: Mukesh Kumar 2025. Triglycerides are an important fuel reserve for synapse function in the brain . protocols.io https://dx.doi.org/10.17504/protocols.io.5qpvooqozv4o/v1
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working.
Created: April 18, 2025
Last Modified: April 18, 2025
Collection Integer ID: 130988
Keywords: DDHD2, Lipid Droplets, Fatty acid oxidation, Torpor
Funders Acknowledgements:
NIH
Grant ID: NS036942
NIH
Grant ID: NS11739
ASAP
Grant ID: ASAP-024404
Abstract
This collection contains all the protocols associated with the study titled 'Triglycerides are an important fuel reserve for synapse function in the brain', published in the 'Nature Metabolism' Journal.
Files
Protocol
Icon representing the file Isolation of Lipid Droplets from Cultured Cortical Neurons
Name
Isolation of Lipid Droplets from Cultured Cortical Neurons
Version 1
, Weill Cornell Medical College
Mukesh KumarWeill Cornell Medical College
Protocol
Icon representing the file Epifluorescence Time-Lapse Imaging of Hippocampal Neurons
Name
Epifluorescence Time-Lapse Imaging of Hippocampal Neurons
Version 1
, Weill Cornell Medical College
Mukesh KumarWeill Cornell Medical College
Protocol
Icon representing the file Electron Microscopy with Primary Hippocampal Neurons
Name
Electron Microscopy with Primary Hippocampal Neurons
Version 1
, Weill Cornell Medical College
Mukesh KumarWeill Cornell Medical College
Protocol
Icon representing the file Fatty Acid Induction to Hippocampal Neurons
Name
Fatty Acid Induction to Hippocampal Neurons
Version 1
, Weill Cornell Medical College
Mukesh KumarWeill Cornell Medical College
Protocol
Icon representing the file Western Blotting for Neuronal Proteins
Name
Western Blotting for Neuronal Proteins
Version 1
, Weill Cornell Medical College
Mukesh KumarWeill Cornell Medical College
Protocol
Icon representing the file Hypothermia Induction in Mice
Name
Hypothermia Induction in Mice
Version 1
, Weill Cornell Medical College
Mukesh KumarWeill Cornell Medical College
Protocol
Icon representing the file Immunocytochemistry with Primary Cultured Hippocampal Neurons
Name
Immunocytochemistry with Primary Cultured Hippocampal Neurons
Version 1
, Weill Cornell Medical College
Mukesh KumarWeill Cornell Medical College
Protocol
Icon representing the file BODIPY 558/568 C12 (Red-C12) Tracing in Hippocampal Neurons
Name
BODIPY 558/568 C12 (Red-C12) Tracing in Hippocampal Neurons
Version 1
, Weill Cornell Medical College
Mukesh KumarWeill Cornell Medical College
Protocol
Icon representing the file Immunohistochemistry with Rodent Brain Sections
Name
Immunohistochemistry with Rodent Brain Sections
Version 1
, Weill Cornell Medical College
Mukesh KumarWeill Cornell Medical College
Protocol
Icon representing the file Lipidomic Analysis of Neuronal Lipid Droplets
Name
Lipidomic Analysis of Neuronal Lipid Droplets
Version 1
, Weill Cornell Medical College
Mukesh KumarWeill Cornell Medical College
Protocol
Icon representing the file shRNA Design, Subcloning, and Lentivirus Production
Name
shRNA Design, Subcloning, and Lentivirus Production
Version 1
, Weill Cornell Medical College
Mukesh KumarWeill Cornell Medical College
Protocol
Icon representing the file Designing Overexpression Plasmids for Cellular Studies
Name
Designing Overexpression Plasmids for Cellular Studies
Version 1
, Weill Cornell Medical College
Mukesh KumarWeill Cornell Medical College