Mar 03, 2025

Public workspaceSynthesis of copper oxide nanoparticles using sodium borohydride as a reducing agent and gelatin as a capping agent V.3

DOI
dx.doi.org/10.17504/protocols.io.q26g7m261gwz/v3
  • 1Center of Excellence in Pharmaceutical Nanotechnology;
  • 2Center of Excellence in Pharmaceutical Nanotechnology, Chiang Mai University,
Mathurada Sasarom
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DOI: dx.doi.org/10.17504/protocols.io.q26g7m261gwz/v3
Protocol Citation: Siriporn Okonogi, Mathurada Sasarom 2025. Synthesis of copper oxide nanoparticles using sodium borohydride as a reducing agent and gelatin as a capping agent. protocols.io https://dx.doi.org/10.17504/protocols.io.q26g7m261gwz/v3Version created by Mathurada Sasarom
Manuscript citation:
Influence of capping agents on the physicochemical properties and leukemic cytotoxicity of copper oxide nanoparticles synthesized using a Caesalpinia sappan plant extract as reducing agent
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 20, 2025
Last Modified: March 03, 2025
Protocol Integer ID: 123642
Keywords: Copper oxide nanoparticles , chemical method, sodium borohydride
Funders Acknowledgements:
The Research and Researcher for Industry
Grant ID: PHD59I0076
Abstract
Copper oxide nanoparticles were synthesized using sodium borohydride as a reducing agent and gelatin as a capping agent according to the method previously described with some slight modifications of method of Liu et al., 2012.
Materials
Materials
1.         Copper sulfate pentahydrate
2.         Gelatin
3.         Sodium borohydride
4.         Sodium hydroxide
5.         Milli-Q water
Equipment
1.         Magnetic stirring
2.         A centrifuge
3.         Sonicator
4.         An oven
Chemical synthesis of gelatin capped copper oxide nanoparticles
Chemical synthesis of gelatin capped copper oxide nanoparticles
10 mL of 0.2 M copper sulfate (or 5.0 g per solution) and 10 mL of 0.1% gelatin dissolved in water were mixed and heated at 70°C for 30 min under magnetic stirring.
10 mL of 0.4 M sodium borohydride (or 0.15 g per solution) was slowly dropped in about 5–10 min into the solution.
The pH of this mixture was adjusted from approximately 2 to 12 by 6 mL of 1 M sodium hydroxide, then heated at 70°C with magnetic stirring for 30 min.
The obtained copper oxide nanoparticles were washed by centrifugation of the nanoparticle dispersion at 8000×g for 30 min.
The obtained pellet was redispersed in 30 mL milli-Q water and this precipitation/washing procedure was repeated two times.
The precipitate was dispersed in absolute ethanol and dried at 60°C for 8 h.
The particles were transferred into light-protecting containers and stored in a desiccator at room temperature until use.
Protocol references
Liu QM, Zhou DB, Yamamoto Y, Ichino R, Okido M. Preparation of Cu nanoparticles with NaBH4 by aqueous reduction method. Trans Nonferrous Met Soc China. 2012;22(1):117–123.