Dec 14, 2021

Public workspaceSM buffer

DOI
dx.doi.org/10.17504/protocols.io.b2tdqei6
  • 1Copenhagen University
  • FOOD Micro UCPH
Frej Larsen
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DOI: dx.doi.org/10.17504/protocols.io.b2tdqei6
Protocol Citation: Frej Larsen 2021. SM buffer. protocols.io https://dx.doi.org/10.17504/protocols.io.b2tdqei6
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it’s working
Created: December 13, 2021
Last Modified: December 14, 2021
Protocol Integer ID: 55877
Abstract
SM buffer is used for diluting and storing bacteriophages.

SM buffer contains:
200 mM NaCl2
10 mM MgSO4
50 mM Tris-HCl, pH 7.5

For storage at -20 or -80° C, 15% glycerol can be added before autoclaving.


The buffer can keep at room temperature for several months.
Guidelines
This recipe is for 1 liter of buffer. Make sure to recalculate the measurements if you are making a smaller or larger volume.

Ensure that your MgSO4 is a monohydrate before weighing. If not, recalculate amount required to reach correct concentration.
Materials
For 1 liter of SM buffer:
11.7 g NaCl2
1.4 g MgSO4 (monohydrate)
50 mL Tris-HCl, pH 7.5 (1 M)
950 mL demineralized water
Weigh off Amount11.7 g NaCl2 and Amount1.4 g MgSO4 and add to a clean 1L blue cap flask or other autoclave safe container.

Measure Amount50 mL Tris-HCl and add to the flask.

Measure Amount950 mL demineralized water and add to the flask.

Screw the cap on tight and mix thoroughly for Duration00:00:30 .

30s
Loosen the cap and autoclave.
If there are still undissolved particles, mix until dissolved. Then let it cool at room temperature.