License: This is an open access collection distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: In development
We are still developing and optimizing this collection.
Created: February 19, 2025
Last Modified: April 01, 2025
Collection Integer ID: 121951
Keywords: EEG, Neuropixels, In Vivo Electrophysiology, Rodent Neurosurgery
Funders Acknowledgements:
Tiny Blue Dot Foundation
Allen Institute
Abstract
This protocol collection details the surgical procedures necessary to record Electroencephalography (EEG) and Neuropixels data simultaneously in a head-fixed mouse. The first step is to perform the "EEG Array and Headframe Implant Surgery". After allowing the mouse to recover for at least 1 week, the next step is to perform the "Burr Hole Craniotomies Through EEG" surgery. Finally, the "External Ground and Reference Strategy for Simultaneous EEG and Neuropixels Recordings" provides information on how to set up and run a recording session.
Image Attribution
Gabriel Rodriguez, Allen Institute
Guidelines
Only perform these procedures in accordance with IACUC and veterinary requirements.
Safety warnings
Personal Protective Equipment (PPE) should be used at all times while operating this protocol.
Isoflurane Warning: Acute over-exposure to waste anesthetic gases (WAG) may cause eye irritation, headache, nausea, drowsiness or dizziness. Repeated exposure may cause damage to cardiovascular system and central nervous system. Refer to MSDS for additional information.
Ethics statement
Research-focused rodent behavior must be conducted according to internationally accepted standards and should always have prior approval from an Institutional Animal Care and Use Committee (IACUC) or equivalent ethics committee(s).
This protocol has been approved by the Allen Institute Animal Care and Use Committee (IACUC).
External Ground and Reference Strategy for Simultaneous EEG and Neuropixels Recordings
Version 1
Lydia C. MarksAllen Institute
Protocol references
Claar L.D., Rembado I., Kuyat J.R., Russo S., Marks L.C., Olsen S.R., Koch C. (2023). Cortico-thalamo-cortical interactions modulate electrically evoked EEG responses in mice. eLife. https://doi.org/10.7554/eLife.84630
Russo S., Claar L.D., Furregoni G., Marks L.C., Krishnan G., , Zauli F.M., Hassan G., Solbiati M., d'Orio P., Mikulan E., Sarasso S., Rosanova M., Sartori I., Bazhenov M., Pigorini A., Massimini M., Koch C., Rembado I. (In Press). Thalamic feedback shapes brain responses evoked by cortical stimulation in mice and humans. Nature Communications.
Russo S., Claar L., Marks L., Krishnan G., Furregoni G., Zauli F.M., Hassan G., Solbiati M., d'Orio P., Mikulan E., Sarasso S., Rosanova M., Sartori I., Bazhenov M., Pigorini A., Massimini M., Koch C., Rembado I. (2024). Thalamic feedback shapes brain responses evoked by cortical stimulation in mice and humans. https://doi.org/10.1101/2024.01.31.578243
