Apr 23, 2025

Public workspaceProtocols for "Sex-dimorphic effects of neuromelanin buildup in rodent nigral dopamine neurons: implications for sex-biased vulnerability in Parkinson’s disease". V.2

DOI
dx.doi.org/10.17504/protocols.io.261gee4kog47/v2
  • 1University of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy;
  • 2Fondazione Santa Lucia IRCCS, Department of Experimental Neuroscience, Rome, Italy;
  • 3Aligning Science Across Parkinson’s (ASAP) Collaborative Research Network;
  • 4Chevy Chase, Maryland USA.;
  • 5Uninettuno Telematic International University, Faculty of Psychology, Rome, Italy;
  • 6Aligning Science Across Parkinson’s (ASAP) Collaborative Research Network, Chevy Chase, Maryland USA.;
  • 7University of Rome Tor Vergata, Department of Systems Medicine, Neurology Unit, Rome, Italy
Ada Ledonne
Icon indicating open access to content
QR code linking to this content
DOI: dx.doi.org/10.17504/protocols.io.261gee4kog47/v2
Collection Citation: Ada Ledonne, daddario.sebastianluca, Nicola Biagio Mercuri 2025. Protocols for "Sex-dimorphic effects of neuromelanin buildup in rodent nigral dopamine neurons: implications for sex-biased vulnerability in Parkinson’s disease".. protocols.io https://dx.doi.org/10.17504/protocols.io.261gee4kog47/v2Version created by Ada Ledonne
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: April 10, 2025
Last Modified: April 23, 2025
Collection Integer ID: 138511
Funders Acknowledgements:
Aligning Science Across Parkinson’s through the Michael J. Fox Foundation for Parkinson’s Research (MJFF)
Grant ID: 020505
MNESYS – A Multiscale integrated approach to the study of the nervous system in health and disease (DN. 1553 11.10.2022) founded by NEXTGENERATIONEU (NGEU) and funded by the Ministry of University and Research (MUR), National Recovery and Resilience Plan (NRRP).
Grant ID: MNESYS (PE0000006)
Abstract
Protocols for "Sex-dimorphic effects of neuromelanin buildup in rodent nigral dopamine neurons: implications for sex-biased vulnerability in Parkinson’s disease".
Files
Protocol
Icon representing the file Stereotaxic virus injection in the SNpc of rats
Name
Stereotaxic virus injection in the SNpc of rats
Version 1
, University of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy
Ada LedonneUniversity of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy
Protocol
Icon representing the file Intracardiac perfusion and rat brain fixation for immunohistochemistry
Name
ForkIntracardiac perfusion and rat brain fixation for immunohistochemistry
Version 1
, University of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy
Ada LedonneUniversity of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy
Protocol
Icon representing the file Immunohistochemistry free-floating rat brain cryosections  
Name
ForkImmunohistochemistry free-floating rat brain cryosections  
Version 1
, University of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy
Ada LedonneUniversity of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy
Protocol
Icon representing the file Immunofluorescence free-floating rat brain cryosections
Name
ForkImmunofluorescence free-floating rat brain cryosections
Version 2
, University of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy
Ada LedonneUniversity of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy
Protocol
Icon representing the file Stereology-mediated cell count using StereoInvestigator
Name
ForkStereology-mediated cell count using StereoInvestigator
Version 2
, University of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy
Ada LedonneUniversity of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy
Protocol
Icon representing the file Masson-Fontana Staining
Name
ForkMasson-Fontana Staining
Version 1
, University of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy
Ada LedonneUniversity of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy
Protocol
Icon representing the file Intracellular neuromelanin quantification
Name
ForkIntracellular neuromelanin quantification
Version 1
, University of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy
Ada LedonneUniversity of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy
Protocol
Icon representing the file Optical densitometry of tyrosine hydroxylase fibers
Name
ForkOptical densitometry of tyrosine hydroxylase fibers
Version 1
, University of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy
Ada LedonneUniversity of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy
Protocol
Icon representing the file Preparation of tissue punches of SNpc for the analyses of mitochondrial function
Name
Preparation of tissue punches of SNpc for the analyses of mitochondrial function
Version 2
, University of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy
Ada LedonneUniversity of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy
Protocol
Icon representing the file Analyses of mitochondrial function in SNpc with the Seahorse XFe96
Name
Analyses of mitochondrial function in SNpc with the Seahorse XFe96
Version 1
, University of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy
Ada LedonneUniversity of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy
Protocol
Icon representing the file Preparation of midbrain slices for electrophysiology
Name
Preparation of midbrain slices for electrophysiology
Version 1
, University of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy
Ada LedonneUniversity of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy
Protocol
Icon representing the file Patch-clamp recordings of SNpc dopamine neurons from midbrain slices
Name
Patch-clamp recordings of SNpc dopamine neurons from midbrain slices
Version 1
, University of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy
Ada LedonneUniversity of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy
Protocol
Icon representing the file Grip strength test
Name
Grip strength test
Version 1
, University of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy
Ada LedonneUniversity of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy
Protocol
Icon representing the file Elevated plus maze test
Name
Elevated plus maze test
Version 1
, University of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy
Ada LedonneUniversity of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy
Protocol
Icon representing the file Marble burying test
Name
Marble burying test
Version 2
, University of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy
Ada LedonneUniversity of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy
Protocol
Icon representing the file Social avoidance test
Name
Social avoidance test
Version 2
, University of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy
Ada LedonneUniversity of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy
Protocol
Icon representing the file Sucrose self-administration protocol
Name
Sucrose self-administration protocol
Version 2
, University of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy
Ada LedonneUniversity of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy
Protocol
Icon representing the file Open field test
Name
Open field test
Version 2
, University of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy
Ada LedonneUniversity of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy
Protocol
Icon representing the file Rotarod test
Name
Rotarod test
Version 1
, University of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy
Ada LedonneUniversity of Rome Tor Vergata, Department of Systems Medicine, Pharmacology Unit, Rome, Italy