Mar 08, 2023

Public workspaceProtocol collection: Sampling and characterizing cheese rind microbial communities

  • 1Arcadia Science
  • Arcadia Science
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Collection CitationAdair Borges, Emily C.P. Weiss 2023. Protocol collection: Sampling and characterizing cheese rind microbial communities. protocols.io https://dx.doi.org/10.17504/protocols.io.n2bvj8j5xgk5/v1
Manuscript citation:
Borges A, Dutton R, McDaniel E, Reiter T, Weiss ECP. (2023). Paired long- and short-read metagenomics of cheese rind microbial communities at multiple time points. https://doi.org/10.57844/arcadia-0zvp-xz86
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: February 27, 2023
Last Modified: January 29, 2024
Collection Integer ID: 77739
Abstract
Cheese rind microbiomes are a validated and stable experimental platform for microbial community research. These communities are relatively simple, reproducible, and easy to grow in the lab. This set of protocols describes how to collect and store cheese rind microbiome samples for isolation of pure cultures, metagenomic sequencing, and other downstream applications. This collection also includes specific protocols for virome extraction and for the isolation of full community and viral-only DNA from cheese samples.
Files
Protocol
Icon representing the file Harvesting and stocking cheese rind community samples
Name
Harvesting and stocking cheese rind community samples
Version 1
, Arcadia Science
Arcadia ScienceArcadia Science
Protocol
Icon representing the file High-molecular-weight DNA extraction from cheese rind microbial communities
Name
High-molecular-weight DNA extraction from cheese rind microbial communities
Version 1
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Arcadia ScienceArcadia Science
Protocol
Icon representing the file Virome harvesting from cheese microbiomes
Name
ForkVirome harvesting from cheese microbiomes
Version 1
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Protocol
Icon representing the file Virome DNA extraction with phenol-chloroform
Name
ForkVirome DNA extraction with phenol-chloroform
Version 1
, Arcadia Science
Arcadia ScienceArcadia Science