Feb 24, 2025

Public workspaceProcessing Serum from Whole Blood

DOI
dx.doi.org/10.17504/protocols.io.kqdg3q121v25/v1
  • Sara Lucas Del Pozo1,2,
  • Kai-Yin Chau1,2,
  • Jane Macnaughtan1,2,
  • Giuseppe Uras1,2,
  • Roxana Mezabrovschi1,2
  • 1University College London;
  • 2Aligning Science Across Parkinson's
Sofia Koletsi
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DOI: dx.doi.org/10.17504/protocols.io.kqdg3q121v25/v1
Protocol Citation: Sara Lucas Del Pozo, Kai-Yin Chau, Jane Macnaughtan, Giuseppe Uras, Roxana Mezabrovschi 2025. Processing Serum from Whole Blood. protocols.io https://dx.doi.org/10.17504/protocols.io.kqdg3q121v25/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 05, 2025
Last Modified: February 24, 2025
Protocol Integer ID: 119992
Keywords: ASAPCRN
Funders Acknowledgements:
Aligning Science Across Parkinson's
Grant ID: ASAP-000420
Abstract
This protocol details the processing of serum from the whole blood sample.
Guidelines
The protocol needs prior approval by the users' Institutional Review Board (IRB), Institutional Animal Care and Use Committee (IACUC) or equivalent ethics committee(s) as applicable.
Safety warnings
The protocol needs prior approval by the users' Institutional Review Board (IRB), Institutional Animal Care and Use Committee (IACUC) or equivalent ethics committee(s) as applicable.
Before start
The protocol needs prior approval by the users' Institutional Review Board (IRB), Institutional Animal Care and Use Committee (IACUC) or equivalent ethics committee(s) as applicable.
Procedure
Procedure
40m
40m
Gently invert the tubes at 180º and back 5-6 times after blood collection. After that, allow the tube to stand upright for Duration00:30:00 minimum from time of collection if needed, to allow for blood clotting to occur.

30m
Centrifuge the yellow cap tube(s) at Centrifigation1800 x g, Room temperature, 00:10:00 NO BRAKE

10m
Centrifigation
Once the centrifugation is complete, the gel must physically separate the red blood cells (bottom layer) and the serum (top layer) as shown in the figure.



Use the Pasteur pipette provided or a micropipette to aliquot Amount250 µL of the serum (upper layer) into the 5 or 6 x 1 ml cryovials labelled as SER.

Pipetting
Place serum samples in Temperature-80 °C .

Note
Processing of the blood needs to be completed within Duration04:00:00 . For waiting periods longer than 2 hours from collection the blood tube should be kept in Temperature0 °C - Temperature4 °C .