May 12, 2023

Public workspaceNCEM Drop - Inactivation of sample on grid (TM-014) V.1

DOI
dx.doi.org/10.17504/protocols.io.8epv59mr6g1b/v1
  • 1CSIRO
sandra.crameri
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DOI: dx.doi.org/10.17504/protocols.io.8epv59mr6g1b/v1
Protocol Citation: sandra.crameri 2023. NCEM Drop - Inactivation of sample on grid (TM-014). protocols.io https://dx.doi.org/10.17504/protocols.io.8epv59mr6g1b/v1Version created by sandra.crameri
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 12, 2023
Last Modified: May 12, 2023
Protocol Integer ID: 81819
Abstract
NCEM Drop - inactivation of samples on grid
Guidelines
This procedure is used for inactivation of 3Z agents, useful if the virus has low titire.
Protocol materials
ReagentGlutaraldehyde aqueous solution 25% EM Grade ampoulesProSciTechCatalog #C002
Reagentnano-WCatalog ##2018-5ML
Adsorb Amount10 µL sample to grid Duration00:10:00

10m
Immerse grid in drop of Concentration2.5 % volume ReagentGlutaraldehyde aqueous solution 25% EM Grade ampoulesProSciTechCatalog #C002 in Concentration0.1 Molarity (M) Sorenson's phosphate buffer Duration00:10:00

10m
Wash Concentration0.1 Molarity (M) Sorenson's phosphate buffer

Using filter paper wick excess buffer from grid - do not dry out, leave grid surface wet.
Stain Reagentnano-WCatalog ##2018-5ML Duration00:01:00

1m
Using filter paper gradually wick stain dry from grid