May 12, 2023

Public workspaceNCEM Drop - Inactivation of sample in solution (TM-014) V.2

DOI
dx.doi.org/10.17504/protocols.io.5jyl89k57v2w/v2
  • 1ACDP CSIRO
sandra.crameri
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DOI: dx.doi.org/10.17504/protocols.io.5jyl89k57v2w/v2
Protocol Citation: sandra.crameri 2023. NCEM Drop - Inactivation of sample in solution (TM-014). protocols.io https://dx.doi.org/10.17504/protocols.io.5jyl89k57v2w/v2Version created by sandra.crameri
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 12, 2023
Last Modified: May 12, 2023
Protocol Integer ID: 81820
Abstract
This inactivation procedure is used to inactivate a sample for Biological Transfer out of PC4, proir to Conventional NCEM.
Protocol materials
ReagentParaformaldehyde (formaldehyde) 16% aqueous solution (DG)ProSciTechCatalog #C004
ReagentParaformaldehyde (formaldehyde) 16% aqueous solution (DG)ProSciTechCatalog #C004
Reagentnano-WCatalog ##2018-5ML
Inactivation
Inactivation
10m
10m
Add Amount50 µL Concentration16 % volume ReagentParaformaldehyde (formaldehyde) 16% aqueous solution (DG)ProSciTechCatalog #C004 to a Sarstedt tube with o-ring (STK#:2056-U, Catalog #61226C2).

Add Amount150 µL sample to the ReagentParaformaldehyde (formaldehyde) 16% aqueous solution (DG)ProSciTechCatalog #C004 already in the tube. Leave contact time Duration00:10:00


10m
Adsorb Amount10 µL sample to grid Duration00:10:00 , inspect to ensure sample doesn't dry out.

10m
Drain excess sample from grid using fliter paper, leave wet.
Stain Reagentnano-WContributed by usersCatalog ##2018-5ML Duration00:01:00

1m
Drain & dry using filter paper