Mar 03, 2025
Nanoparticle Tracking Analysis (NTA)
- Emily Tabaie1
- 1University of California, Riverside
Protocol Citation: Emily Tabaie 2025. Nanoparticle Tracking Analysis (NTA). protocols.io https://dx.doi.org/10.17504/protocols.io.dm6gp9761vzp/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 03, 2025
Last Modified: March 03, 2025
Protocol Integer ID: 123713
Abstract
This protocol is designed to identify the average size and concentration of particles using a nanoparticle tracking analysis (NTA). These steps are to be done immediately after the isolation of extracellular vesicles.
Nanoparticle Tracking Analysis (NTA)
Nanoparticle Tracking Analysis (NTA)
Turn on the machine and tighten in the 4 beige screws into the glass chamber
Place the glass chamber into the machine and move up the red lever to lock into place
Grab a new 1 mL syringe and wash the machine with 1 mL of distilled water 2-3 times until no particles are visible
Attach the syringe to the injection port and fix it in the injection port
Go to capture (left top corner) -> click start camera
Go to interface -> hardware (on the right) -> syringe pump (on the top) -> click infuse
Scroll up and down to see the brightness of the particles passing on the screen
Once the wash is done grab that same 1 mL syringe and take up all 1 mL of your sample
Insert the syringe and state infusion
Wait until the volume on the syringe reaches 300-400 ul and start the analysis
Go SOP (left) -> recent measurement (top) -> double click on generic triplicate (will show the script panel on the left) -> click on file name -> click on D drive -> Ziting -> Emily -> create a folder with the date
Hit run -> creator name (Emily) -> diluent (100) -> sample description (whatever the sample is) -> okay -> settings okay
Hit settings okay -> export -> click on the folder -> drive -> folder -> results -> pdf -> export to USB drive
Take the syringe and inject into the waste and throw the syringe into the red biohazard bag
Get a new syringe and insert 1 mL of distilled water into the syringe pump to wash the pump and repeat once
Go to hardware -> syringe pump -> infuse
When all the samples are complete wash the chamber with a total of 3 mL distilled water
Clean the glass chamber with ethanol, turn off the machine, and close the program