Primer | Sequence (5'-3') | |
RSAB1F | TTCTTGTTAGGTGTWGGATCTGCAAT | |
RSAB1R | GTTACACCTGCATTRACACTRAATTC | |
RP-F | GACTTCAGCATGGCGGTGT | |
RP-R | GAGCGGCTGTCTCCACAAGT |
Probe | Sequence (5'-3') | |
RSVA-FAM | TCTACTATCCACAAACAAGGCTGTAGTC | |
RSVB-HEX | TTCCAACAATCTGCTGTTCTTCTGCTGG | |
RP-TxRd | TTCTGACCTGAAGGCTCTGCGCG |
Reagent | Use [] | 1x (ul) | |
H2O | 5.25 | ||
2x PCR Master Mix | 12.5 | ||
RSAB1F Primer | 40 uM | 0.5* | |
RSAB1A Primer | |||
RP-F Primer | 20 uM | 0.5* | |
RP-R Primer | |||
RSV A-FAM Probe | 10 uM | 0.25 | |
RSV B-HEX Probe | 0.25 | ||
RP-TxRd | 2.5 uM | 0.25 | |
SuperScript III RT/Platinum | 0.5 |
A | B | C | |
52ºC | 30 min | ||
95ºC | 2 min | ||
95ºC | 15 seg | 50x | |
55ºC | 1 min 30 seg |