Feb 21, 2025
Version 3
Mouse Brain Perfusion and Flash Freezing V.3
- Allen Institute1
- 1Allen Institute
- Allen Institute for Brain ScienceTech. support
Protocol Citation: Allen Institute 2025. Mouse Brain Perfusion and Flash Freezing . protocols.io https://dx.doi.org/10.17504/protocols.io.j8nlkodr6v5r/v3Version created by Allen Institute
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: September 27, 2023
Last Modified: February 21, 2025
Protocol Integer ID: 123226
Abstract
This protocol is used for transcardial perfusion with HEPES-Sucrose Cutting Solution (Referred as HEPES hereafter), followed by fast brain dissection and flash freezing.
Note: Research reported in this publication was supported by the National Institute Of Mental Health of the National Institutes of Health under Award Number U19MH114830. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
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