Apr 07, 2025
Molecular Cartography/Resolve OCT Sectioning
- 1Stanford University
- Human BioMolecular Atlas Program (HuBMAP) Method Development CommunityTech. support email: Jeff.spraggins@vanderbilt.edu
Protocol Citation: Joanna Bi 2025. Molecular Cartography/Resolve OCT Sectioning. protocols.io https://dx.doi.org/10.17504/protocols.io.bp2l68bxrgqe/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: April 07, 2025
Last Modified: April 07, 2025
Protocol Integer ID: 126300
Abstract
Protocol for sectioning OCT samples for Molecular Cartography/Resolve.
Set cryostat to -20° C. Place samples inside to acclimate to temperatures for 10 minutes.
Add OCT media to the chuck and place the sample block on top. Let freeze for 5 minutes.
Insert and fasten cryostat blade and set sectioning thickness to 30-60 um.
Place chuck in specimen station and adjust sample distance and angle to blade.
Trim sample block until desired depth is reached.
Lightly score block with a razor blade so that tissue fits within the designated scan areas on the 8-sample Resolve slides.
Section at 5 um and place section within the a scan area of the slide.
Repeat as needed to fill the slide.
Store slides at -80°C directly after sectioning.