Protocol Citation: Olha Kunakh, Hanna Tutova, Olena Lisovets, Olexander Zhukov 2025. Methods for assessing the temporal dynamics of landscape cover based on procrustean analysis of spectral indices. protocols.io https://dx.doi.org/10.17504/protocols.io.n92ld59k7v5b/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Comparisons in different time periods using individual spectral indices
are often used to assess the dynamics of vegetation cover. The NDVI is a very
popular index for indicating disturbances in the structure of vegetation cover.
Various vegetation indices are highly correlated for homogeneous vegetation
cover, so assessing dynamics based on one of them is quite appropriate.
Similarly, other single spectral indices can be used for other homogeneous
surface types, such as agricultural land, rocks, or water. However, in the case
of complicated landscape complexes, there is a need to assess the dynamics of
heterogeneous land cover types using indices that are sensitive to the
characteristics of vegetation, soil and water surface. Anthropogenic pressure
also strongly affects the physiological state of plants, so it is also
appropriate to use spectral indices that are sensitive to plant health. The
multidimensionality of the spectral index space raises the problem of reducing
the dimensionality of this space, as well as comparing different landscape
states over time. The method uses principal component analysis to reduce the
dimensionality of the spectral index space and Procrustes analysis to compare
the structures established by the results of principal component analysis. The
R code uses as an example an image of the surface of the southern part of
Khortytsia Island, where floodplain ecosystems are located, which were
negatively affected by the environmental disaster caused by the undermining of
the Kakhovka Dam by the Russian occupiers in 2023. Accordingly, the case study
compares the state of floodplain ecosystems in 2022 and 2024.
General characteristics of spectral indices
General characteristics of spectral indices
The Kakhovka Dam was destroyed, resulting in the subsequent devastation
of the Kakhovka Reservoir on June 6, 2023. To evaluate the changes in landscape
cover in the affected area, we analyzed images of the southern part of
Khortytsia Island and the surrounding waters of the Dnipro River, taken one
year before the disaster (August 24, 2022) and one year after the disaster
(August 18, 2024). We utilized Sentinel-2B Level-2A imagery, which includes 13
spectral channels (B1 – B12, including B8A) and has a resolution of 10 meters:
B2 (Blue), B3 (Green), B4 (Red), B8 (NIR); 20 meters: B5-B7, B8A, B11, B12; and
60 meters: B1, B9, B10. For further analysis, the images were reclassified to a
resolution of 10 meters. We characterized the landscape cover features using 29
spectral indices (Table 1). The most commonly used
channel for calculating spectral indices was B4 (red, 665 nm), which was
utilized to compute 17 out of 29 indices, accounting for 58.6%. This channel is
essential for classical NDVI-like indices that evaluate green biomass, pigment
composition, and vegetation productivity. B8 (NIR, 842 nm) was employed to
calculate 15 indices (51.7%). This channel is primarily used for detecting
vegetation due to the high reflectivity of plants in the near-infrared range.
The B2 (blue, 490 nm) and B3 (green, 560 nm) channels were used to compute 12
(41.4%) and 11 (37.9%) indices, respectively. These channels are important for
detecting water stress, pigment imbalance, and spectral contrast. Red-edge
channels are also widely utilized: B5 (705 nm) and B7 (783 nm) are included in
11 indices (37.9% each), while B6 (740 nm) is included in 7 indices (24.1%).
This underscores the significance of the red-edge range for accurately
determining chlorophyll concentration, plant physiological status, and early signs
of stress. SWIR channels, which reflect soil moisture, salinity, and surface
structural properties, also exhibit a high frequency of use: B11 (1610 nm) was
employed in 10 indices (34.5%), and B12 (2190 nm) in 8 indices (27.6%). Less
frequently, B1 (coastal aerosol, 443 nm) was used in 3 indices (10.3%), and B8A
(narrow NIR, 865 nm) in 2 indices (6.9%). These channels serve highly
specialized functions, such as assessing atmospheric impurities (B1) or
providing detailed spectral detection of chlorophyll (B8A). The overall
structure of channel usage frequency reveals a dominance of indices focused on
assessing total phytomass, water stress, moisture, and soil conditions, which
are critical for monitoring the state of natural cover and anthropogenically
transformed landscapes. The high frequency of use of the B4 (665 nm), B8 (842
nm), B2 (490 nm), and B3 (560 nm) channels highlights the key role of
traditional NDVI-like indices, while the inclusion of red-edge (705–783 nm) and SWIR (1610–2190 nm) channels enhances the accuracy of assessments regarding the
physiological state of plants and soils.
Table 1. Spectral Indices for Ecological Analysis
Table 1. Spectral Indices for Ecological Analysis
A
B
C
D
E
Aerosol Contrast Index
AC_Index
(B2 – B1) /
(B1 +
B2)
This spectral index is applied for detailed examination of coastal and
inland waters. It is capable of observing sediments, particles, organic
matter and chlorophyll-rich phytoplankton in these waters.
(Komlyk
et al., 2024)
Blue-Green Index
BIG2
B2 / B3
BIG2 distinguishes between vegetation and water or soil; sensitive to
pigment concentration.
(Elfanah
et al., 2023)
Blue-normalized difference vegetation index
BNDVI
(B12 – B1) /
(B12 + B1)
The BNDVI is a modification of the standard NDVI, where a blue channel
is used instead of the red channel (Red). Its sensitivity is more focused on
low chlorophyll levels, which allows it to better detect stressed or degraded
plants. The use of the blue channel makes the index more stable in cases of
noise pollution or shadows, which is especially important in the early stages
of plant growth.
(Yang
et al., 2004)
Green Leaf Index
GLI
(2 * B3 – B4 – B2)
/ (2 * B3 + B4 + B2)
Indicates vegetation density and photosynthetic activity. The index
ranges from -1 to +1. A negative value indicates soil or dead cover, while a
positive value indicates green leaves and stems. A threshold with a value
close to 0 divides the image into two classes: green leaves and soil or
non-living cover.
(Louhaichi
et al., 2001)
Green NDVI
GNDVI
(B7 – B3) /
(B7 + B3)
Highly sensitive to chlorophyll content; used in vegetation stress
monitoring.
(Gitelson
et al., 1996)
Index Name
Abbreviation
Formula
Ecological Meaning
Reference
Land Surface Water Index
LSWI
(B5 – B6) /
(B5 + B6)
Reflects water content in vegetation and soil; used for drought
monitoring.
(Chandrasekar
et al., 2010)
Modified NDWI
MNDW
(B3 – B11) /
(B3 + B11)
Enhanced water body extraction by replacing NIR with SWIR.
(Xu,
2006)
Normalized Burn Ratio Index
NBRI
(B8 – B12) /
(B8 + B12)
Detects burned areas and vegetation loss due to fires.
(Seydi
et al., 2021)
Normalized Difference Bareness Index
NDBaI
(B6 – B11) /
(B6 + B11)
Extracts bare soil areas automatically.
(Zhao
and Chen, 2005)
Normalized Difference Chlorophyll-a
NDChla
(B3 – B4) /
(B3 + B4)
Estimates chlorophyll-a concentration in water.
(Ha
et al., 2017)
Normalized Difference Green Chlorophyll Index
NDGCI
(B8a – B3) /
(B8a + B3)
Estimates leaf chlorophyll content, indicator of plant health.
(Chaves
et al., 2020)
Normalized Difference Index
NDI
(B12 – B7) /
(B12 + B7)
Used to estimate soil salinity.
(Wang
et al., 2019)
Normalized Difference Infrared Index
NDII
(B8 – B11) /
(B8 + B11)
Indicates water content in vegetation; used for drought assessment.
(Hardisky
et al., 1983)
Normalized Difference Iron Oxide
NDIO
(B4 – B2) /
(B2 + B4)
Detection of ferric iron oxides.
(Yazdi
et al., 2013)
Normalized Difference Red Edge Index
NDRE
(B7 – B5) /
(B7 + B5)
NDRE is a modified version of NDVI that uses red-edge instead of red
band. Red-edge is a spectral region between red and NIR (~700-740 nm) that is
sensitive to chlorophyll content, even in dense vegetation. The index does
not saturate at high biomass, unlike NDVI. It is a better indicator of
chlorophyll, especially in the later stages of crop growth, when NDVI is no
longer sensitive. Sensitive to nitrogen stress - as chlorophyll content is
often directly related to nitrogen content in plants. It is used to assess
stress associated with water and nitrogen deficiencies. Reliable for partial
soil coverage - less dependent on cover density than other indices. It has a
high correlation with the nitrogen content in the leaves, surpassing other
vegetation indices in this regard.
(Barnes
et al., 2000)
Normalized Difference Red-Edge Improved
NDREI
(B8 – B5) /
(B8 + B5)
NDREI is an improved version of the NDRE index, which uses RE1 (B6 or
B7) instead of RE2 (B5), as well as near-infrared (NIR). The choice of RE1 +
NIR provides better spectral sensitivity to early changes in chlorophyll and
plant condition. The index reduces saturation, which is typical for NDVI, and
responds better to light plant stress, even with a high leaf area index
(LAI). The index is highly sensitive to chlorophyll concentration. NDREI
accurately reflects chlorophyll even in dense cover. It is optimal for
assessing nitrogen supply. It is particularly effective in crops where
nitrogen is closely related to chlorophyll content. Less dependent on cover
density than NDVI or GNDVI. Increased stability when changing the sun angle
or atmospheric conditions due to the use of a red-edge channel closer to the
NIR. Effective for monitoring late vegetation stages when NDVI becomes less
sensitive.
(Barnes
et al., 2000)
Normalized Difference Tillage Index
NDTI
(B11 – B12) /
(B11 + B12)
The index uses two SWIR channels that are sensitive to soil moisture
content and surface structure. The NDTI index detects the difference between
treated and untreated soil based on their different reflectivity in the SWIR
range. Changes in the surface structure (e.g. after ploughing or harrowing)
affect the spectral response that this index captures. High ability to
distinguish between tillage methods, e.g. no-till, minimum-till, conventional
tillage. It is resistant to changes in vegetation cover and is particularly
effective in areas with no or minimal vegetation. It can be used to assess
erosion vulnerability, as the surface structure directly affects erosion
processes.
(Van
Deventer et al., 1997)
Normalized Difference Total Suspended Matter Index
NDTSM
(B7 – B2) /
(B7 + B2)
Estimates total suspended matter in water bodies.
(Premkumar
et al., 2021)
Normalized Difference Vegetation Index
NDVI
(B8 – B4) /
(B8 + B4)
Indicates vegetation productivity and greenness. One of the most
popular vegetation indices.
(Rouse
et al., 1974)
Normalized Difference Water Index 1
NDWI1
(B3 – B8) /
(B3 + B8)
NDWI1 exploits the difference in spectral reflectivity between water
and land in the green and NIR bands. Positive values (close to +1): indicate
open water. Zero or negative values are typical for vegetation and soils. The
main application is to identify lakes, rivers, and reservoirs in images.
NDWI1 can be an indicator of overall turbidity.
(McFeeters,
1996)
Normalized Difference Water Index 2
NDWI2
(B5 – B12) /
(B5 + B12)
NDWI₂ is sensitive to the moisture content of soil and vegetation. It
is primarily applied to assess water stress in plants and soil moisture
levels. Low NDWI₂ values indicate drought or reduced moisture. The NDWI₂ is
not intended to detect water as much as the classic NDWI1, but to indicate
the moisture in the environment. NDWI₂ is often used under the name NDMI
(Normalised Difference Moisture Index).
(Gašparović
and Singh, 2020)
Red Edge Difference Index
REDI
(B7 – B6) /
(B7 + B6)
The index is highly sensitive to changes in chlorophyll. The index
compares two adjacent red-edge bands, where subtle changes in the slope of
the spectral curve are recorded as chlorophyll content increases or
decreases. The index also has low sensitivity to soil and water background. A
narrow spectral range between channels B6 and B7 minimises the influence of
background components, particularly in the case of incomplete vegetation
coverage. In dense vegetation, this index is less prone to saturation than classical
vegetation indices. It can be used for high-precision analyses of the
dynamics of changes in vegetation condition due to its spectral stability.
The index is sensitive to changes in chlorophyll content, so it is efficient
for detecting nutritional stress, aging, and pest damage.
(Addabbo
et al., 2016)
Red Edge NDVI 1
RedEdge_NDVI1
(B6 – B4) /
(B6 +
B4)
This index is a variant of NDVI, where instead of NIR, red-edge, which
is sensitive to chlorophyll, is used, and instead of the traditional green,
red, which is highly absorbed by pigments, is used. The red-edge range
(680-750 nm) is characterised by a sharp transition between chlorophyll
absorption and mesophyll reflection. B6 is particularly sensitive to early
changes in chlorophyll, making it useful for detecting subtle or initial
changes in vegetation. Red-edge reflectance is predominantly driven by chlorophyll
content, not just leaf area, which allows for an assessment of the
physiological state of plants. Red Edge NDVI 1 displays LAI changes much more
accurately than classic NDVI. It allows you to identify the stages of plant
development, initial signs of stress, leaf aging or nutrient deficiencies.
(Xie
et al., 2018)
Red Edge NDVI 2
RedEdge_NDVI2
(B7 – B4) /
(B7 + B4)
This index is a modified NDVI based solely on the red-edge spectrum.
It can be considered a highly specialised index for assessing the
physiological state of plants, in particular chlorophyll content. The index
is particularly sensitive to chlorophyll concentration in leaves and less
sensitive to soil background. Even small changes in the structure or
concentration of pigments can significantly affect the index value. Red-edge
NDVI 2 does not reach a plateau as quickly at high LAI values, which allows for
better distinction of dense crowns. Relatively less dependent on water
content: this index focuses more specifically on photosynthetic pigments.
(Xie
et al., 2018)
Red Edge Normalized Difference Vegetation Index
RENDVI
(B7 – B5) /
(B7 +
B5)
Unlike the classical NDVI, which is based on the red and NIR bands,
RENDVI uses two red-edge channels, which gives high sensitivity to
chlorophyll concentration without saturation under high LAI conditions. The
index is particularly effective in detecting plant stress, nitrogen
deficiency, and aging. Since the reflections in the red-edge are formed
mainly by leaves, the index is less sensitive to the brightness of the litter
layer than the classical indices. It shows changes in chlorophyll content
even under early stress, before visual symptoms appear. Suitable for
analysing the dynamics of medium and dense vegetation in time series.
(Gitelson
et al., 1996)
Red–Blue NDVI
RBNDVI
(B8 – (B5 + B1)) /
(B8 + B5 + B1)
A three-component index derived from NDVI can better assess mixed
vegetation and soil conditions. The B1 (coastal aerosol) channel is sensitive
to aerosols and dust, so its inclusion in the denominator allows for
normalisation of the effect of atmospheric influence on reflectance. The B5
red-edge channel provides a better assessment of vegetation stress
conditions, including early signs of wilting, dryness, or salinity. The
integration of information on soil, vegetation and atmosphere makes the index
more stable in arid and semi-arid regions, where other indices have a lower
correlation with real indicators of land cover. It is used for monitoring
soil salinity, assessing vegetation cover in saline environments, and has
improved discriminatory ability to distinguish between vegetation, bare soil
and saline areas.
(Wang
et al., 2019)
Rock Index
RI
(B3 – B12) /
(B3 +
B12)
Index based on the contrast of visible and SWIR reflectance. The green
band (B3) is well reflected by most illuminated surfaces (including rocks),
while the SWIR band (B12) is sensitive to moisture, clay minerals, carbonates
and sulphates. Rocks with high reflectivity in the visible spectrum but low
SWIR reflectivity (quartzite, flint) give high positive values. Mixed
moistened clay and altered rocks (with hydrothermal influence) exhibit lower
or negative values. The index is analogous to NDVI for geological purposes.
However, RI characterises rock surface types instead of vegetation. It is
used to identify rock outcrops, especially in open, dry regions. High RI
values (closer to +1) indicate dry, reflective surfaces (light rocks,
quartzites). Low RI values (closer to -1) may indicate wet, hydrothermally
altered areas or dense vegetation.
(Imbroane
et al., 2007)
Sentinel–2 Vegetation Salinity Index
SVSI
(B4 – B2) /
(B5 + B11)
The index assesses the difference between the visual absorption of
pigments (chlorophyll, carotenoids) in the blue/red range (B2, B4) and the
combined contribution of leaf structure and water (B5 + B11). The index
reveals the secondary effects of soil salinity primarily through a decrease
in chlorophyll, a decrease in water content, and damage to leaf structure.
Indicates the accumulation of Cl- and Na⁺ ions in plant leaves through their
spectral effect on pigment composition and moisture content. It allows to
assess the level of plant stress in saline areas.
(Lugassi
et al., 2017)
Structure Insensitive Pigment Index
SIPI
(B8 – B2) /
(B8 –
B4)
SIPI is developed to minimise the influence of structural
characteristics of the plant cover (e.g. leaf area, leaf distribution) on the
assessment of plant pigment composition. SIPI increases with decreasing
relative chlorophyll content and increasing carotenoids, and is therefore a
marker of stress, aging, and leaf yellowing. SIPI ≈ 1.0 indicates a high
chlorophyll content and low stress level. SIPI > 1.2 indicates an increase
in the proportion of carotenoids, possible degradation or stress. SIPI
effectively reflects changes in the pigment balance due to water deficiency,
pathogens, and nutrient deficiencies. It is used to monitor the degradation
of stands in forests and parks.
(Penuelas
et al., 1995)
Vegetation indexes
Vegetation indexes
The group of indices, including NDVI, GNDVI, GLI, NDGCI, NDRE, RedEdge
NDVI1/2, and RENDVI, effectively reflect both quantitative (density, Leaf Area
Index [LAI]) and qualitative (pigment content, physiological state of plants)
aspects of vegetation cover. Their application enables the monitoring of
productivity, stress, and degradation of phytomass over extensive areas,
particularly exemplified by the landscape changes following the destruction of
the Kakhovka reservoir.
Spectral indices are sensitive to soil cover properties
Spectral indices are sensitive to soil cover properties
Among the spectral indices utilized in this study, several specialized
indices enable to characterize the properties of soil cover, including its
bareness, surface structure, moisture content, salinity, and iron oxide levels.
Notably, the Normalized Difference Bare Soil Index (NDBaI) automatically
identifies areas with bare soil, serving as an indicator of vegetation
degradation or active erosion processes. The Normalized Difference Tillage
Index (NDTI), which employs two shortwave infrared (SWIR) channels, is
sensitive to moisture content and changes in micro-relief. This sensitivity allows
it to differentiate between treated and untreated soil and to identify
structures that increase the risk of erosion. The NDI index reflects the degree of salinity at the soil surface, which
is particularly important for monitoring degraded areas or those transformed by
irrigation. The Rock Index (RI) differentiates between surface types based on
the ratio of visible to shortwave infrared (SWIR) reflectance, which can be
useful for identifying mineral differences, especially in arid and open
regions. The NDIO index characterizes the presence of ferrous oxides, enabling
the assessment of soil chemistry and signs of hydrothermal changes. The NDWI2,
NDII, and LSWI indices, although traditionally used to evaluate the moisture
content of vegetation, are also sensitive to soil moisture, particularly in
bare areas where infrared wavelengths dominate the spectral response. The selected
indices facilitate a comprehensive assessment of soil cover, considering its
physical structure, moisture content, chemical composition, and degree of
bareness. This is critical for detecting degradation processes, spatial
heterogeneity, and evaluating anthropogenic impacts on ecosystems.
Spectral indices are sensitive to the properties of the water environment
Spectral indices are sensitive to the properties of the water environment
Among the spectral indices utilized in this study,
several indicators are crucial for characterizing the ecological conditions of
the aquatic environment, as they reflect both the presence of open water and
the quality of aquatic vegetation cover. These indices include the Normalized
Difference Water Index 1 (NDWI1), which facilitates the identification of water
surfaces by contrasting the green and near-infrared spectral ranges. Its
modification, the Modified Normalized Difference Water Index (MNDWI), enhances
water separation in the presence of aquatic vegetation or suspended solids by
utilizing the Short-Wave Infrared (SWIR) channel. Concurrently, the Land
Surface Water Index (LSWI), the Normalized Difference Infrared Index (NDII),
and NDWI2 (also known as the Normalized Difference Moisture Index, NDMI) are
sensitive to the moisture content of vegetation and soil, making them widely
used for assessing water stress levels, particularly in agroecosystems and
wetlands. Additionally, the NDTSM facilitates the estimation of suspended
solids concentration in water, a crucial indicator of turbidity and
anthropogenic load. The NDChla index is employed to approximate chlorophyll-a
content, which may signify levels of eutrophication or substantial
phytoplankton development. A distinct category encompasses indices related to
saline environmental conditions, such as SVSI and NDI, which reflect the
secondary effects of salinity through reductions in pigment content and
alterations in vegetation structure. Another significant index is the RBNDVI, a
three-component metric that integrates the characteristics of atmospheric
exposure, vegetation, and soil salinity. Finally, the AC_Index enables the
estimation of aerosol and organic matter content in coastal waters, which is
essential for pollution detection. Thus, spectral indices designed to assess
the aquatic environment provide a comprehensive overview of its condition,
including humidity, the presence of water bodies, pollution levels,
eutrophication, and salinity. This information is vital for monitoring changes
in the context of hydrological and anthropogenic transformations.
Statistical analysis features
Statistical analysis features
Spectral indices for reducing the dimensionality of the trait space were subjected to
principal component analysis (Eastman and Fulk, 1993). Primary principal
component analysis (PCA) typically identifies dominant patterns that reveal the
most significant features of landscape cover structure, such as the ratio of
vegetated to non-vegetated areas and the ratio of land to water. These land cover
types are markedly distinct from one another and occupy a substantial portion
of the imagery. When land cover changes are not widespread—such as forest fires
or the complete destruction of water bodies—general patterns of landscape
structure dominate the variability in the feature space, obscuring
smaller-scale land cover changes. To detect these smaller-scale spatial
patterns, the variability associated with principal components 1 and 2 was
extracted from the spectral index values. This was accomplished by calculating
linear regressions of the spectral indices against PC 1 and PC 2. The residuals
from these regression models were then subjected to secondary principal
component analysis. The results of the PCA on the residuals were further
analyzed using Procrustes analysis with the use of the vegan library (Oksanen
et al., 2022). The procrustes function from this library rotates one
configuration to the maximum similarity with another configuration minimizing
sum of squared differences. The protest function tests for nonrandomness
(significance) between two configurations. Procrustes rotation is typically
used in comparison of ordination results. In this case, the solutions obtained
from the analysis of the principal components of the residuals of the regression
dependencies of spectral indices on the primary principal components are
compared.
# Екстрагуємо значення для кожного пункту. Функція повертає
data.frame,
# де перший стовпець – ідентифікатор точки, а інші –
значення відповідно до входжень у кожен шар.
# Extract
values for each point. The function returns a data.frame where the first column
is the point ID and the others are the values from each layer.
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