Apr 22, 2020

Public workspaceLight microscopic analysis of synaptic input to neurons in the rat major pelvic ganglion [keast-003]

DOI
dx.doi.org/10.17504/protocols.io.bakiicue
  • 1University of Melbourne
  • SPARC
    Tech. support email: info@neuinfo.org
Janet R Keast
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DOI: dx.doi.org/10.17504/protocols.io.bakiicue
Collection Citation: Janet R Keast, Peregrine B Osborne 2020. Light microscopic analysis of synaptic input to neurons in the rat major pelvic ganglion [keast-003]. protocols.io https://dx.doi.org/10.17504/protocols.io.bakiicue
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: December 16, 2019
Last Modified: November 26, 2023
Collection Integer ID: 31082
Keywords: Immunohistochemistry, immunofluorescence, retrograde tracing, tract tracing, synapse
Abstract
This collection describes the procedures required to visualise and characterize synaptic boutons associated with functionally classified pelvic ganglion autonomic neurons of adult male and female Sprague-Dawley rats. This collection includes protocols for:

STAGE 1: Surgery to micro-inject fluorescent retrograde tracer dyes into one or more sites within the lower urinary tract

STAGE 2: Intracardiac perfusion with fixative to preserve neural tissues of interest

STAGE 3: Immunohistochemical labeling of thick cryosections of pelvic ganglia

STAGE 4: Confocal microscopy and image analysis of synaptic boutons associated with ganglion neurons.
Files
Protocol
Icon representing the file Use of tracer dyes to label neural projections to lower urinary tract organs
Name
Use of tracer dyes to label neural projections to lower urinary tract organs
Version 1
, University of Melbourne
Janet R KeastUniversity of Melbourne
Protocol
Icon representing the file Intracardiac perfusion with fixative for anatomical studies
Name
ForkIntracardiac perfusion with fixative for anatomical studies
Version 1
, University of Melbourne
Janet R KeastUniversity of Melbourne
Protocol
Icon representing the file Immunohistochemical labeling of thick cryosections from pelvic ganglia
Name
ForkImmunohistochemical labeling of thick cryosections from pelvic ganglia
Version 1
, University of Melbourne
Janet R KeastUniversity of Melbourne
Protocol
Icon representing the file Confocal microscopy and characterization of synaptic boutons associated with ganglion neurons
Name
Confocal microscopy and characterization of synaptic boutons associated with ganglion neurons
Version 1
, University of Melbourne
Janet R KeastUniversity of Melbourne