Feb 17, 2025

Public workspaceJaworski's Media

This protocol is a draft, published without a DOI.
  • Katelyn Brown1
  • 1Bowling Green State University
Katelyn Brown
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Protocol Citation: Katelyn Brown 2025. Jaworski's Media. protocols.io https://protocols.io/view/jaworski-39-s-media-dzx577q6
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
Created: February 17, 2025
Last Modified: February 17, 2025
Protocol Integer ID: 120541
Abstract
Protocol for making Jaworski's Media (JM) that is used for culturing freshwater cyanobacteria.
Protocol materials
ReagentCalcium nitrate tetrahydrateBio Basic Inc.Catalog #CB0258.SIZE.500g
ReagentPotassium phosphate (monobasic)P212121
ReagentSodium nitrateP212121
ReagentMilli-Q water
ReagentMagnesium sulfate heptahydrateMerck MilliporeSigma (Sigma-Aldrich)Catalog #M2773
ReagentSodium phosphate dibasicMerck MilliporeSigma (Sigma-Aldrich)Catalog #7558-79-4
ReagentSodium bicarbonateP212121
ReagentEDTA Disodium SaltCatalog #PubChem CID: 8759
ReagentBoric acidP212121
ReagentManganese(II) chloride tetrahydrateMerck MilliporeSigma (Sigma-Aldrich)Catalog #M3634
ReagentFerric-sodium EDTAMerck MilliporeSigma (Sigma-Aldrich)
ReagentAmmonium molybdate (VI) tetrahydrateFisher ScientificCatalog # 12054-85-2
ReagentMilli-Q water
ReagentMilli-Q water
ReagentVitamin B12Fisher ScientificCatalog #68-19-9
ReagentThiamine HClP212121
ReagentBiotinP212121
ReagentMilli-Q water
Jaworski's Media
Jaworski's Media
Stock solution #1 Temperature4 °C
Amount200 mL ReagentMilli-Q waterContributed by users
Amount4 g ReagentCalcium nitrate tetrahydrateBio Basic Inc.Catalog #CB0258.SIZE.500g
Amount2.48 g ReagentPotassium phosphate (monobasic)P212121
Amount10 g ReagentMagnesium sulfate heptahydrateMerck MilliporeSigma (Sigma-Aldrich)Catalog #M2773
Amount3.18 g ReagentSodium bicarbonateP212121
Amount16 g ReagentSodium nitrateP212121
Amount7.2 g ReagentSodium phosphate dibasicMerck MilliporeSigma (Sigma-Aldrich)Catalog #7558-79-4

  • Autoclave
Stock solution #2 Temperature4 °C
Amount200 mL ReagentMilli-Q waterContributed by users
Amount0.45 g ReagentFerric-sodium EDTAMerck MilliporeSigma (Sigma-Aldrich)
Amount0.45 g ReagentEDTA Disodium SaltContributed by usersCatalog #PubChem CID: 8759
Amount0.496 g ReagentBoric acidP212121
Amount0.278 g ReagentManganese(II) chloride tetrahydrateMerck MilliporeSigma (Sigma-Aldrich)Catalog #M3634
Amount0.20 g ReagentAmmonium molybdate (VI) tetrahydrateFisher ScientificCatalog # 12054-85-2

  • Autoclave
Vitamins stock solution Temperature-20 °C
Amount200 mL ReagentMilli-Q waterContributed by users
Amount8 mg ReagentVitamin B12Fisher ScientificCatalog #68-19-9
Amount8 mg ReagentThiamine HClP212121
Amount8 mg ReagentBiotinP212121

Preparation
Amount1 L ReagentMilli-Q waterContributed by users
Amount1 mL Stock solution #1
Amount1 mL Stock solution #1
Amount10 µL Vitamins stock solution

  • Autoclave
Protocol references