Mar 06, 2020
- 1Hainan University
External link: https://doi.org/10.1371/journal.pone.0234435
Protocol Citation: Jing Xu 2020. Hydroxyl Radical-scavenging Activity. protocols.io https://dx.doi.org/10.17504/protocols.io.bdazi2f6
Manuscript citation:
Zhou J, Yang Q, Zhu X, Lin T, Hao D, Xu J (2020) Antioxidant activities of Clerodendrum cyrtophyllum Turcz leaf extracts and their major components. PLoS ONE 15(6): e0234435. doi: 10.1371/journal.pone.0234435
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 06, 2020
Last Modified: March 06, 2020
Protocol Integer ID: 33849
Abstract
The scavenging of hydroxyl radicals were determined by Guo et al. The reactions were performed in 0.3 ml of 20 mM sodium salicylate, 2.0 ml of 1.5 nM FeSO4, 1.0 ml of samples, and 1.0 ml of 6 mM H2O2.The reaction mixture was incubated for 1 hat 37 °C. The absorbance was measured at 510 nm. The inhibition percent and 50% of absorbance(EC50)were calculated.