Jun 06, 2023

Public workspaceCylinder behavior test

DOI
dx.doi.org/10.17504/protocols.io.eq2ly7m9qlx9/v1
  • Hong-Yuan Chu1,2
  • 1Van Andel Institute;
  • 2ASAP
Johnson Agniswamy
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DOI: dx.doi.org/10.17504/protocols.io.eq2ly7m9qlx9/v1
Protocol Citation: Hong-Yuan Chu 2023. Cylinder behavior test. protocols.io https://dx.doi.org/10.17504/protocols.io.eq2ly7m9qlx9/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: May 31, 2023
Last Modified: May 31, 2024
Protocol Integer ID: 82671
Keywords: Cylinder behavior test, ASAPCRN
Funders Acknowledgements:
ASAP
Grant ID: ASAP-020572
Abstract
This protocol describes cylinder behavior test.
Attachments
Icon for 728-1839.docx
728-1839.docx
15KB
Materials
Apparatus

  1. A 600-ml glass beaker
  2. A digital camcorder at 60 fps (Panasonic, HC-V180K)
Procedure
Procedure
Move the test mice from their housing room to the testing room and allow to acclimate for around Duration02:00:00 .
2h
Keep the test environment under dim white-light illumination (around 150 lux).
Place two mirrors perpendicular to each other to form a ninety-degree angle.
Clean a 600-ml glass beaker with 70% ethanol and water before the test.
Place the 600-ml glass beaker in front of the two mirrors.
Place a digital camcorder in front of the 600-ml glass beaker towards the glass beaker and mirrors.
Note
Ensure the glass cylinder is not overlapping in the mirror.

Place a test mouse into the 600-ml glass beaker and start recording.
After 10 minutes, stop recording and return the test mouse immediately to its home cage.
Clean the 600-ml glass beaker with 70% ethanol and water.
Analysis
Analysis
Analyze videos offline by a person blinded to the treatments.
Give a score of “left forepaw”, “right forepaw”, or “both” based on which paw was used during a rear.
  1. If the animal walked up the wall during a rear, that counted as “both”. At the end, “both” was divided equally between “left paw” and “right paw”.
  2. Only one point was given per rear. Another point was not given until the animal returned to all fours and reared again.
  3. The paw must be splayed out and pressed against the glass cylinder to count as a point – resting the paw gently does not count.

Sum up the total number of touches for each forepaw and determine the percentage used by dividing the number of touches for each forepaw by the total number of touches.