Feb 12, 2025

Public workspaceCollection of protocols related to Article - Proteostasis and lysosomal repair deficits in transdifferentiated neurons of Alzheimer's disease V.2

DOI
dx.doi.org/10.17504/protocols.io.kxygxw4wwv8j/v2
  • 1Stanford University
Ching-Chieh Chou
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DOI: dx.doi.org/10.17504/protocols.io.kxygxw4wwv8j/v2
Collection Citation: Ching-Chieh Chou, Judith Frydman 2025. Collection of protocols related to Article - Proteostasis and lysosomal repair deficits in transdifferentiated neurons of Alzheimer's disease. protocols.io https://dx.doi.org/10.17504/protocols.io.kxygxw4wwv8j/v2Version created by Ching-Chieh Chou
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: February 11, 2025
Last Modified: February 12, 2025
Collection Integer ID: 120151
Keywords: ASAPCRN
Funders Acknowledgements:
Aligning Science Across Parkinson's
Grant ID: ASAP-000282
Aligning Science Across Parkinson's
Grant ID: ASAP-024268
Abstract
A collection of protocols used in the study.
Files
Protocol
Icon representing the file Human primary fibroblast culture
Name
Human primary fibroblast culture
Version 1
, Stanford University
Ching-Chieh ChouStanford University
Protocol
Icon representing the file Neuronal transdifferentiation from human primary adult fibroblasts
Name
Neuronal transdifferentiation from human primary adult fibroblasts
Version 1
, Stanford University
Ching-Chieh ChouStanford University
Protocol
Icon representing the file Lentivirus preparation for neuronal transdifferentiation
Name
Lentivirus preparation for neuronal transdifferentiation
Version 1
, Stanford University
Ching-Chieh ChouStanford University
Protocol
Icon representing the file Lentivirus preparation for neuronal transdifferentiation
Name
Lentivirus preparation for neuronal transdifferentiation
Version 2
, Stanford University
Ching-Chieh ChouStanford University
Protocol
Icon representing the file Immunohistochemistry on free-floating and paraffin-embedded tissue sections
Name
Immunohistochemistry on free-floating and paraffin-embedded tissue sections
Version 1
, Stanford University
Ching-Chieh ChouStanford University
Protocol
Icon representing the file Cytokine profiling analysis on conditioned medium of human neurons using Luminex multiplex assay
Name
Cytokine profiling analysis on conditioned medium of human neurons using Luminex multiplex assay
Version 1
, Stanford University
Ching-Chieh ChouStanford University
Protocol
Icon representing the file Caspase-3/7 activity
Name
Caspase-3/7 activity
Version 1
, Stanford University
Ching-Chieh ChouStanford University
Protocol
Icon representing the file Lysosomal and mitochondrial functional assays in human neurons
Name
Lysosomal and mitochondrial functional assays in human neurons
Version 1
, Stanford University
Ching-Chieh ChouStanford University
Protocol
Icon representing the file Aβ ELISA
Name
Aβ ELISA
Version 1
, Stanford University
Ching-Chieh ChouStanford University
Protocol
Icon representing the file Transmission electron microscopy
Name
Transmission electron microscopy
Version 1
, Stanford University
Ching-Chieh ChouStanford University
Protocol
Icon representing the file Perfusion/ Fixation for cryostat slicing for HHC
Name
Perfusion/ Fixation for cryostat slicing for HHC
Version 1
, Trudeau Lab
Nicolas GiguèreTrudeau Lab
Protocol
Icon representing the file CSF Processing
Name
CSF Processing
Version 1
, Brigham and Women's Hospital and Harvard Medical School
Daniel El KodsiBrigham and Women's Hospital and Harvard Medical School
Protocol
Icon representing the file LC-MS/MS Analysis
Name
LC-MS/MS Analysis
Version 1
, Northwestern University, Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, MD 20815
Chuyu ChenNorthwestern University, Aligning Science Across Parkinson's (ASAP) Collaborative Research Network, Chevy Chase, MD 20815
Protocol
Icon representing the file Multiplexed TMTpro proteomic sample preparation of whole cell HeLa lysates from various growth conditions ± FAC
Name
ForkMultiplexed TMTpro proteomic sample preparation of whole cell HeLa lysates from various growth conditions ± FAC
Version 1
,
Felix Kraus