Mar 17, 2023
CARD COUNT PROTOCOL
- 1UC Berkeley
Protocol Citation: Grace Sandel, Sage Moloney 2023. CARD COUNT PROTOCOL. protocols.io https://dx.doi.org/10.17504/protocols.io.5qpvor1nbv4o/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: March 16, 2023
Last Modified: March 17, 2023
Protocol Integer ID: 78940
Abstract
Procedure to conduct the card count portion of "Crazy Yellow Ant Sampling on Tetiaroa"
The point of this protocol is to quantify the prevalence of yellow crazy ants in given transects around Tetiaroa.
Guidelines
- Do not trespass
- Analyze site safety before conducting a sample
- Avoid trampling flora
- If a point is inside a building, just go to the next point or as close to the point as possible
Materials
White flashcard (10cm x 10cm)
Stopwatch
EpiCollect5 mobile app
Safety warnings
If it starts raining, stop deploying vials and card counts.
Before start
Follow this protocol after arriving at the sample site coordinates (ex: D5, E17).
Do card counts before deploying vials.
Card Count Protocol
Card Count Protocol
If needed, scrape the ground (at least a 10x10cm area) at the site so the card sits flat on the ground (i.e. if there are branches or leaf litter, scrape them away).
If you scraped the ground, wait 30 seconds before placing the card down. If you did not scrape the ground, place the card down without waiting.
Place the card down on the ground.
For 30 seconds, count the number of yellow crazy ants that cross the card.
After 30 seconds have passed, pick up the card and remove all ants from it.
Enter the number of ants counted in the card count into the appropriate field of the EpiCollect5 form.
Protocol references
Jayna Devore - Crazy Yellow Ant Sampling on Tetiaroa