Feb 18, 2025

Public workspaceBG-11 Media

This protocol is a draft, published without a DOI.
  • Katelyn Brown1
  • 1Bowling Green State University
Katelyn Brown
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Protocol Citation: Katelyn Brown 2025. BG-11 Media. protocols.io https://protocols.io/view/bg-11-media-dzyg77tw
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
Created: February 17, 2025
Last Modified: February 18, 2025
Protocol Integer ID: 120552
Abstract
Protocol for making BG-11 media that is used for culturing freshwater cyanobacteria.
Protocol materials
ReagentPotassium phosphate (dibasic)P212121
ReagentMagnesium sulfate heptahydrateMerck MilliporeSigma (Sigma-Aldrich)Catalog #M2773
ReagentCalcium Chloride DihydrateFisher ScientificCatalog #C79
ReagentSodium carbonateMerck MilliporeSigma (Sigma-Aldrich)Catalog #223484
ReagentMilli-Q water
ReagentMilli-Q water
ReagentCitric AcidP212121
ReagentEDTA disodium dihydrateAbblisCatalog #AB1011793
ReagentFerric citrateMerck MilliporeSigma (Sigma-Aldrich)
ReagentMilli-Q water
ReagentSodium molybdate dihydrate
ReagentCopper (II) sulfate pentahydrateMerck MilliporeSigma (Sigma-Aldrich)Catalog #209198
ReagentCobalt (II) nitrate hexahydrateFisher ScientificCatalog # 10026-22-9
ReagentZinc sulfate heptahydrateMerck MilliporeSigma (Sigma-Aldrich)Catalog #204986
ReagentBoric acidP212121
ReagentManganese chloride, tetrahydrateBio Basic Inc.Catalog #MB0331.SIZE.1Kg
ReagentMilli-Q water
ReagentVitamin B12Fisher ScientificCatalog #68-19-9
ReagentMilli-Q water
ReagentBiotinP212121
ReagentMilli-Q water
ReagentMilli-Q water
ReagentThiamine HClP212121
BG-11 Media
BG-11 Media
Stock solution #1 Temperature4 °C
Amount1 L ReagentMilli-Q waterContributed by users
Amount30 g ReagentPotassium phosphate (dibasic)P212121
Amount75 g ReagentMagnesium sulfate heptahydrateMerck MilliporeSigma (Sigma-Aldrich)Catalog #M2773
Amount36 g ReagentCalcium Chloride DihydrateFisher ScientificCatalog #C79
Amount20 g ReagentSodium carbonateMerck MilliporeSigma (Sigma-Aldrich)Catalog #223484
Stock solution #2 Temperature4 °C
Amount1 L ReagentMilli-Q waterContributed by users
Amount1 g ReagentEDTA disodium dihydrateAbblisCatalog #AB1011793
Amount6 g ReagentFerric citrateMerck MilliporeSigma (Sigma-Aldrich)
Amount6 g ReagentCitric AcidP212121
Vitamin primary stock solutions Temperature-20 °C
Vitamin B12
Amount5 mL ReagentMilli-Q waterContributed by users
Amount5 mg ReagentVitamin B12Fisher ScientificCatalog #68-19-9
Biotin
Amount10 mL ReagentMilli-Q waterContributed by users
Amount1 mg ReagentBiotinP212121
Vitamin working stock solution Temperature-20 °C
Amount100 mL ReagentMilli-Q waterContributed by users
Amount0.1 mL Vitamin B12 stock solution
Amount0.1 mL Biotin stock solution
Amount0.02 g ReagentThiamine HClP212121
Trace metals stock solution Temperature4 °C
Amount1 L ReagentMilli-Q waterContributed by users
Amount2.86 g ReagentBoric acidP212121
Amount1.81 g ReagentManganese chloride, tetrahydrateBio Basic Inc.Catalog #MB0331.SIZE.1Kg
Amount0.222 g ReagentZinc sulfate heptahydrateMerck MilliporeSigma (Sigma-Aldrich)Catalog #204986
Amount0.390 g ReagentSodium molybdate dihydrateContributed by users
Amount0.079 g ReagentCopper (II) sulfate pentahydrateMerck MilliporeSigma (Sigma-Aldrich)Catalog #209198
Amount0.0494 g ReagentCobalt (II) nitrate hexahydrateFisher ScientificCatalog # 10026-22-9
Preparation
  • Amount1 L ReagentMilli-Q waterContributed by users
  • Amount1 mL Stock solution #1
  • Amount1 mL Stock solution #2
  • Amount1 mL Vitamin working stock solution
  • Amount1 mL Trace metals stock solution
  • Ph7.5 Adjust pH
  • Autoclave
Protocol references