Mar 04, 2025

Public workspaceAnalyzing degranulation in HMC1.2 and RBL-2H3 cells

  • 1Arcadia Science
  • Arcadia Science
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Collection CitationJustin Donnelly 2025. Analyzing degranulation in HMC1.2 and RBL-2H3 cells. protocols.io https://dx.doi.org/10.17504/protocols.io.5jyl8dqr7g2w/v1
Manuscript citation:
Borges AL, Donnelly J, Morazan E, Rollins M. (2025). Compound 48/80 is toxic in HMC1.2 and RBL-2H3 cells. https://doi.org/10.57844/arcadia-3207-4695
License: This is an open access collection distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this collection and it's working
Created: January 28, 2025
Last Modified: March 13, 2025
Collection Integer ID: 119252
Abstract
A collection detailing procedures for culturing, activating, and analyzing the degranulation of mast cells. Also included is a procedure to assess the impact of activators on cell viability, which is a crucial control.
Files
Protocol
Icon representing the file Culturing HMC1.2 human mast cells
Name
Culturing HMC1.2 human mast cells
Version 1
, Arcadia Science
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Protocol
Icon representing the file Culturing RBL-2H3 rat basophils
Name
Culturing RBL-2H3 rat basophils
Version 1
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Arcadia ScienceArcadia Science
Protocol
Icon representing the file Treating mast cells and basophils with degranulation inducers
Name
Treating mast cells and basophils with degranulation inducers
Version 1
, Arcadia Science
Arcadia ScienceArcadia Science
Protocol
Icon representing the file Detecting degranulation via hexosaminidase assay
Name
Detecting degranulation via hexosaminidase assay
Version 1
, Arcadia Science
Arcadia ScienceArcadia Science
Protocol
Icon representing the file Quantifying cell viability via LDH cytotoxicity assay
Name
Quantifying cell viability via LDH cytotoxicity assay
Version 1
, Arcadia Science
Arcadia ScienceArcadia Science