Feb 20, 2025

Public workspace"Olympus Slideview VS200" slide scanning

DOI
dx.doi.org/10.17504/protocols.io.8epv52qr6v1b/v1
  • 1VHIR-CIBERNED-ASAP
  • Vilalab Public
Miquel Vila
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DOI: dx.doi.org/10.17504/protocols.io.8epv52qr6v1b/v1
Protocol Citation: Gerard Roch Alba 2025. "Olympus Slideview VS200" slide scanning . protocols.io https://dx.doi.org/10.17504/protocols.io.8epv52qr6v1b/v1
License: This is an open access protocol distributed under the terms of the Creative Commons Attribution License,  which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited
Protocol status: Working
We use this protocol and it's working
Created: February 19, 2025
Last Modified: February 20, 2025
Protocol Integer ID: 121705
Abstract
Brief guide on the main steps to follow in order to scann a sample with the "Olympus Slideview VS200 slide scanner. Although examples for certain parameters are presented, each user should choose them based on the characteristics of their samples.
Preparation
Preparation
Turn on the Olympus Slideview VS200 slide scanner hardware and associated software.
Click on "exchange trays".
Setting scan parameters
Setting scan parameters
Click on "single scann" or "batch scann" depending on your needs. The batch scann does not allow manual focusing.
Click on "edit scann settings". Select the objective and magnification. The 40x objective requires immersion oil, which is automatically applied by the scanner.
Click on "detail". Set the Z-range, Z-space and Z-count as needed. In case of a 20μm-thick section, for instance: Z-range = 12μm , Z-space = 2,95 μm, and Z-count = 5.
Select the focus mode. In case of a strong stainning, the authomatic method (and thus the batch scan) can be used, while a weak stainning may require semi-automatic/ manual focusing.
Click on "Naming and saving". Name the scan/s and select the folder where they wil be saved upon completion.
Start the pre-scan. Once it is complete, click on "select scan area". Draw the scan areas around the regions of interest. Then click on "select focus map" and establish the focus points within the scan areas.
Focusing and scanning
Focusing and scanning
Start scan.
If the "manual focusing option" was previously selected, use the control+scroller to adjust the focus. Turning the "Auto-contrast" option on can help with challanging slides. Once all the points have been focused, start scan.
Saving
Saving
Click on "save and home" or "save and new scan" based on your needs.